ASTM E3226-19
(Test Method)Standard Test Method for Processing Cellulose Sponge-wipes to Detect Bacillus anthracis Spores Sampled from Environmental Surfaces
Standard Test Method for Processing Cellulose Sponge-wipes to Detect <emph type="ital" >Bacillus anthracis</emph> Spores Sampled from Environmental Surfaces
SIGNIFICANCE AND USE
5.1 This procedure describes a standardized method of processing cellulose wipes in a biosafety level 3 laboratory in order to detect and provide a semi-quantitative estimate of B. anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of contamination or for clearance of an area after decontamination.
SCOPE
1.1 This test method covers a standardized method of processing cellulose wipes in a biosafety level 3 (BSL3) laboratory in order to detect and provide a semi-quantitative estimate of Bacillus anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of the contamination, or for area clearance after decontamination.
1.2 The laboratory procedures should be performed in a BSL3 laboratory by those trained for BSL3 microbiological techniques.
1.3 This test method is specific to B. anthracis, but could be adapted for use with other organisms.
1.4 The interlaboratory study was conducted with cellulose sponge wipes pre-moistened with neutralizing buffer. All reproducibility, sensitivity, and specificity data are based on the performance of these wipes. A review was conducted by subcommittee in 2019, and re-confirmed these ILS data are valid.
1.5 Units—The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
Relations
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3226 − 19
Standard Test Method for
Processing Cellulose Sponge-wipes to Detect Bacillus
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anthracis Spores Sampled from Environmental Surfaces
This standard is issued under the fixed designation E3226; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
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2.1 ASTM Standards:
1.1 This test method covers a standardized method of
E177Practice for Use of the Terms Precision and Bias in
processing cellulose wipes in a biosafety level 3 (BSL3)
ASTM Test Methods
laboratory in order to detect and provide a semi-quantitative
E691Practice for Conducting an Interlaboratory Study to
estimate of Bacillus anthracis contamination after sampling of
Determine the Precision of a Test Method
non-porous surfaces. Sampling may be conducted to charac-
E2756Terminology Relating toAntimicrobial andAntiviral
terize the extent of the contamination, or for area clearance
Agents
after decontamination.
1.2 The laboratory procedures should be performed in a
3. Terminology
BSL3 laboratory by those trained for BSL3 microbiological
3.1 Fordefinitionsofgeneraltermsusedinthistestmethod,
techniques.
refer to E2756 Terminology Relating to Antimicrobial and
1.3 Thistestmethodisspecificto B. anthracis,butcouldbe Antiviral Agents
adapted for use with other organisms.
3.2 Definitions:
3.2.1 accuracy, n—the closeness of the agreement between
1.4 The interlaboratory study was conducted with cellulose
theresultofameasurementandatruevalueofthequantitythat
sponge wipes pre-moistened with neutralizing buffer. All
is being measured.
reproducibility,sensitivity,andspecificitydataarebasedonthe
performance of these wipes. A review was conducted by
3.2.2 eluate, n—aneluent,whichmaycontaintherecovered
subcommittee in 2019, and re-confirmed these ILS data are organism (s).
valid.
3.3 Definitions of Terms Specific to This Standard:
3.3.1 eluent, n—any solution that is harmless to the organ-
1.5 Units—The values stated in SI units are to be regarded
ism(s) recovered from a surface, and that is added to a wipe to
as standard. The values given in parentheses after SI units are
recover the organism(s) from it.
providedforinformationonlyandarenotconsideredstandard.
3.3.2 laboratory response network (LRN), n—a national
1.6 This standard does not purport to address all of the
network of local, state and federal public health, food testing,
safety concerns, if any, associated with its use. It is the
veterinary diagnostic, and environmental testing laboratories
responsibility of the user of this standard to establish appro-
that provide the laboratory infrastructure and capacity to
priate safety, health, and environmental practices and deter-
respond to biological and chemical terrorism, and other public
mine the applicability of regulatory limitations prior to use.
health emergencies.
1.7 This international standard was developed in accor-
3.3.3 non-porous, adj—describes a surface that is resistant
dance with internationally recognized principles on standard-
to absorption of liquid.
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom- 3.3.4 quality control (QC), v—the operational techniques
mendations issued by the World Trade Organization Technical and the activities, which sustain a quality of material, product,
Barriers to Trade (TBT) Committee. system, or service that will satisfy given needs; also the use of
such techniques and activities.
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This test method is under the jurisdiction of ASTM Committee E35 on
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Pesticides, Antimicrobials, and Alternative Control Agents and is the direct For referenced ASTM standards, visit the ASTM website, www.astm.org, or
responsibility of Subcommittee E35.15 on Antimicrobial Agents. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved Oct. 1, 2019. Published November 2019. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/E3226–19 the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E3226 − 19
3.3.4.1 Discussion—The quality control techniques and ac- 4.4 Asemi-quantitative estimate of the amount of contami-
tivities are to ensure the precision of the method, and identify nation is derived based upon growth on the plates, on the filter
if any cros
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