EN ISO 6887-1:2017

Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)

ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption.
ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result.
ISO 6887-1:2017 excludes preparation of samples for both enumeration and detection test methods where preparation instructions are detailed in specific International Standards.

Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen (ISO 6887-1:2017)

Dieses Dokument legt allgemeinen Regeln für die aerobe Herstellung von Erstverdünnungen und Verdünnungen für mikrobiologische Untersuchungen von Produkten fest, die für den Verzehr durch Menschen oder Tiere vorgesehen sind.
Dieses Dokument gilt für den allgemeinen Fall und andere Teile gelten für spezifische Produkt¬gruppen, wie im Vorwort dargelegt. Einige Aspekte könnten auch auf molekulare Verfahren anwendbar sein, bei denen Matrices mit der Hemmung von PCR Schritten in Zusammenhang stehen und folglich das Untersuchungsergebnis beeinflussen können.
Dieses Dokument schließt die Vorbereitung von Proben für Untersuchungsverfahren zur Zählung und zum Nachweis aus, bei denen die Anweisungen für die Vorbereitung in spezifischen Internationalen Normen ausführlich dargelegt sind.

Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales (ISO 6887-1:2017)

ISO 6887-1:2017 définit des règles générales pour la préparation de la suspension mère et des dilutions réalisées en aérobiose, en vue des examens microbiologiques des produits destinés à la consommation humaine ou à l'alimentation animale.
ISO 6887-1:2017 est applicable aux cas généraux et les autres parties s'appliquent aux groupes de produits spécifiques indiqués dans l'Avant-propos. Certains aspects peuvent aussi être applicables aux méthodes moléculaires dans lesquelles les matrices peuvent être impliquées dans l'inhibition des étapes de PCR et, de ce fait, affectent le résultat d'essai.
ISO 6887-1:2017 exclut la préparation d'échantillons en vue des méthodes de dénombrement et de recherche dans lesquelles les instructions de préparation sont détaillées dans des Normes internationales spécifiques.

Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne suspenzije in decimalnih razredčin (ISO 6887-1:2017)

Ta dokument določa splošna pravila za aerobno pripravo osnovne suspenzije in razredčin za mikrobiološke preiskave izdelkov, namenjenih za prehrano ljudi ali živali. Ta dokument se uporablja za splošne primere, drugi deli pa veljajo za posebne skupine izdelkov, kot je omenjeno v predgovoru. Nekateri vidiki se lahko uporabljajo tudi za molekularne metode, kjer je mogoče matrice povezati z zaviranjem verižne reakcije s polimerazo, kar vpliva na rezultate preskusa. Ta dokument ne zajema priprave vzorcev za preskusni metodi za ugotavljanje števila in odkrivanje, kadar so navodila glede priprave podrobneje opisana v določenih mednarodnih standardih.

General Information

• Status: Published
• Publication Date: 04-Apr-2017
• ICS: 07.100 - Microbiology
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 05-Apr-2017
• Completion Date: 05-Apr-2017
• Ref Project: SIST EN ISO 6887-1:2017 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)

Relations

• Replaces: EN ISO 6887-1:1999 - Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:1999)
• Effective Date: 12-Apr-2017
• Amended By: EN ISO 6887-1:2017/FprA1 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative method (ISO 6887-1:2017/FDAM1:2024)
• Effective Date: 23-Jun-2021

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 6887-1:2017
01-junij-2017
1DGRPHãþD
SIST EN ISO 6887-1:1999
0LNURELRORJLMDYSUHKUDQVNLYHULJL3ULSUDYDSUHVNXVQLKY]RUFHYRVQRYQH
VXVSHQ]LMHLQGHFLPDOQLKUD]UHGþLQ]DPLNURELRORãNRSUHLVNDYRGHO6SORãQD
SUDYLOD]DSULSUDYRRVQRYQHVXVSHQ]LMHLQGHFLPDOQLKUD]UHGþLQ,62
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen (ISO 6887-1:2017)
Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des
dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales
pour la préparation de la suspension mère et des dilutions décimales (ISO 6887-1:2017)
Ta slovenski standard je istoveten z: EN ISO 6887-1:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6887-1:2017 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 6887-1:2017

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SIST EN ISO 6887-1:2017


EN ISO 6887-1
EUROPEAN STANDARD

NORME EUROPÉENNE

April 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 6887-1:1999
English Version

Microbiology of the food chain - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions
(ISO 6887-1:2017)
Microbiologie de la chaîne alimentaire - Préparation Mikrobiologie der Lebensmittelkette - Vorbereitung
des échantillons, de la suspension mère et des dilutions von Untersuchungsproben und Herstellung von
décimales en vue de l'examen microbiologique - Partie Erstverdünnungen und von Dezimalverdünnungen für
1: Règles générales pour la préparation de la mikrobiologische Untersuchungen - Teil 1: Allgemeine
suspension mère et des dilutions décimales (ISO 6887- Regeln für die Herstellung von Erstverdünnungen und
1:2017) Dezimalverdünnungen (ISO 6887-1:2017)
This European Standard was approved by CEN on 14 January 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-1:2017 E
worldwide for CEN national Members.

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SIST EN ISO 6887-1:2017
EN ISO 6887-1:2017 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 6887-1:2017
EN ISO 6887-1:2017 (E)
European foreword
This document (EN ISO 6887-1:2017) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall
be withdrawn at the latest by October 2017.
Attention is drawn to the possibility that some of the elements of this document may
...

SLOVENSKI STANDARD
oSIST prEN ISO 6887-1:2014
01-februar-2014
0LNURELRORJLMDYSUHKUDQVNLYHULJL3ULSUDYDY]RUFHY]DSUHVNXãDQMHWHURVQRYQHLQ
GHFLPDOQLKUD]UHGþLQ]DPLNURELRORãNRSUHLVNDYRGHO6SORãQDSUDYLOD]D
SULSUDYRRVQRYQHLQGHFLPDOQLKUD]UHGþLQ,62',6
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions (ISO/DIS 6887-1:2013)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen (ISO/DIS 6887-1:2013)
Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des
dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales
pour la préparation de la suspension mère et des dilutions décimales (ISO/DIS 6887-
1:2013)
Ta slovenski standard je istoveten z: prEN ISO 6887-1
ICS:
07.100.30 Mikrobiologija živil Food microbiology
oSIST prEN ISO 6887-1:2014 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 6887-1:2014

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oSIST prEN ISO 6887-1:2014
DRAFT INTERNATIONAL STANDARD
ISO/DIS 6887-1
ISO/TC 34/SC 9 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2013-11-28 2014-04-28
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
Microbiologie de la chaîne alimentaire — Préparation des échantillons, de la suspension mère et des
dilutions décimales en vue de l’examen microbiologique —
Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales
[Revision of first edition (ISO 6887-1:1999)]
ICS: 07.100.30
ISO/CEN PARALLEL PROCESSING
This draft has been developed within the International Organization for
Standardization (ISO), and processed under the ISO lead mode of collaboration
as defined in the Vienna Agreement.
This draft is hereby submitted to the ISO member bodies and to the CEN member
bodies for a parallel five month enquiry.
Should this draft be accepted, a final draft, established on the basis of comments
received, will be submitted to a parallel two-month approval vote in ISO and
THIS DOCUMENT IS A DRAFT CIRCULATED
formal vote in CEN.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
To expedite distribution, this document is circulated as received from the
IN ADDITION TO THEIR EVALUATION AS
committee secretariat. ISO Central Secretariat work of editing and text
BEING ACCEPTABLE FOR INDUSTRIAL,
composition will be undertaken at publication stage.
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 6887-1:2013(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2013

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oSIST prEN ISO 6887-1:2014
ISO/DIS 6887-1:2013(E)

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oSIST prEN ISO 6887-1:2014
ISO/DIS 6887-1
Contents Page
Foreword . v
Introduction .
...