ASTM E1153-22

Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate, Hard, Nonporous Non-Food Contact Surfaces

SIGNIFICANCE AND USE
4.1 This test method shall be used to determine if a chemical intended for use as a non-food contact sanitizer or as a one-step cleaner-sanitizer provides percent reductions of the selected test organisms on treated carriers as compared to control.
SCOPE
1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous, non-food contact surfaces against Staphylococcus aureus, or Klebsiella pneumoniae or Klebsiella aerogenes, or a combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When utilizing test surfaces not described herein (see Test Method E2274) or when evaluating spray-based or towelette-based antimicrobial products, modifications may also be required.  
1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner-sanitizer formulations recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces.  
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.)  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard may involve hazardous materials, chemicals and microorganisms and should be performed only by persons who have had formal microbiological training.  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 30-Sep-2022
• ICS: 11.080.20 - Disinfectants and antiseptics
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1153 − 22
Standard Test Method for
Efficacy of Sanitizers Recommended for Inanimate, Hard,
1
Nonporous Non-Food Contact Surfaces
This standard is issued under the fixed designation E1153; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
1.1 This test method is used to evaluate the antimicrobial
efficacy of sanitizers on precleaned, inanimate, hard,
2. Referenced Documents
nonporous, non-food contact surfaces against Staphylococcus
2
2.1 ASTM Standards:
aureus,or Klebsiella pneumoniaeor Klebsiella aerogenes,ora
D1193Specification for Reagent Water
combination thereof. Appropriate modifications to the method
E1054PracticesforEvaluationofInactivatorsofAntimicro-
may be required when testing organisms not specified herein.
bial Agents
When utilizing test surfaces not described herein (see Test
E2274Test Method for Evaluation of Laundry Sanitizers
Method E2274) or when evaluating spray-based or towelette-
and Disinfectants
based antimicrobial products, modifications may also be re-
E2756Terminology Relating toAntimicrobial andAntiviral
quired.
Agents
1.2 This test method may also be used to evaluate the
2.2 Federal Standard:
3
antimicrobial efficacy of one-step cleaner-sanitizer formula-
40CFR, Part 160, Good Laboratory Practice Standards
tions recommended for use on lightly soiled, inanimate,
nonporous, non-food contact surfaces. 3. Terminology
1.3 It is the responsibility of the investigator to determine 3.1 Terms used in this test method are defined in Terminol-
whether Good Laboratory Practices (GLP) are required and to ogy E2756.
follow them where appropriate (see section 40 CFR, 160 or as
3.2 Definitions of Terms Specific to This Standard:
revised.)
3.2.1 accuracy, n—ameasureofthedegreeofconformityof
1.4 The values stated in SI units are to be regarded as a value generated by a specific procedure to the assumed or
standard. No other units of measurement are included in this
accepted true value, and includes both precision and bias.
standard.
3.2.2 ambient temperature, n—temperature of the environ-
1.5 This standard may involve hazardous materials, chemi-
ment in which a test method is performed.
cals and microorganisms and should be performed only by
3.2.3 antimicrobial, adj—describes an agent that kills or
persons who have had formal microbiological training. This
inactivates microorganisms or suppresses their growth or
standard does not purport to address all of the safety concerns,
reproduction.
if any, associated with its use. It is the responsibility of the user
3.2.4 bias, n—a systematic error that contributes to the
of this standard to establish appropriate safety, health, and
difference between the mean of a large number of test results
environmental practices and determine the applicability of
and an accepted reference value.
regulatory limitations prior to use.
3.2.5 cleaner-sanitizer, n—a physical or chemical agent that
1.6 This international standard was developed in accor-
removes soil from an object and reduces numbers of microor-
dance with internationally recognized principles on standard-
ganisms on non-food contact surfaces.
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom- 3.2.6 carrier, n—a surrogate surface or matrix that facili-
tates the interaction of test microorganisms and treatment(s).
1 2
This test method is under the jurisdiction of ASTM Committee E35 on For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
responsibility of Subcommittee E35.15 on Antimicrobial Agents. Standards volume information, refer to the standard’s Document Summary page on
Current edition approved Oct. 1, 2022. Published October 2022. Originally the ASTM website.
ε1
3
approved 1987. Last previous edition approved in 2014 as E1153–03(2014) . Available from the Superintendent of Documents, U.S. Government Printing
DOI: 10.1520/E1153-22. Office, Washington, DC 20402.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E11
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
´1
Designation: E1153 − 14 E1153 − 22
Standard Test Method for
Efficacy of Sanitizers Recommended for Inanimate, Hard,
1
Nonporous Non-Food Contact Surfaces
This standard is issued under the fixed designation E1153; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1
ε NOTE—Section 13.3.2 was editorially corrected in June 2020.
1. Scope Scope*
1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous,
non-food contact surfaces against Staphylococcus aureus, or Klebsiella pneumoniae or EnterobacterKlebsiella aerogenes, or a
combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When
utilizing test surfaces not described herein (see Test Method E2274) or when evaluating spray-based or towelette-based
antimicrobial products, modifications may also be required.
1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner-sanitizer formulations
recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow
them where appropriate (see section 40 CFR, 160 or as revised.)
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard may involve hazardous materials, chemicals and microorganisms and should be performed only by persons
who have had formal microbiological training. This standard does not purport to address all of the safety concerns, if any,
associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental
practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E1054 Practices for Evaluation of Inactivators of Antimicrobial Agents
E2274 Test Method for Evaluation of Laundry Sanitizers and Disinfectants
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2014Oct. 1, 2022. Published May 2014October 2022. Originally approved 1987. Last previous edition approved in 20102014 as
ε1
E1153 – 03(2010)(2014) . DOI: 10.1520/E1153-14E01.10.1520/E1153-22.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E1153 − 22
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
2.2 Federal Standard:
3
40 CFR, Part 160, Good Laboratory Practice Standards
3. Terminology
3.1 Terms used in this test method are defined in Terminology E2756.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 accuracy, n—a measure of the degree of conformity of a value generated by a specific procedure to the assumed or accepted
true value, and includes both precision and bias.
3.2.2 ambient temperature, n—temperature of the environment in which a test method is performed.
3.2.3 antimicrobial, adj—describes an agent that kills or inactivates microorganisms or suppresses their growth or reproduction.
3.2.4 bias, n—a systematic error that contributes to the difference between the mean of a large number of test results and an
accepted reference value.
3.2.5 cleaner-sanitizer, n—a physical or chemical agent that removes soil from an object and reduces
...