Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography

This document method is applicable for the determination of theobromine in compound feed by liquid chromatography with UV detection in the tested range of 27 to 307 mg/kg. This method has been validated using complementary compound feed for adult dogs and complementary compound feedstuff for horses. The actual working range may extend beyond the tested range. Alternative chromatography conditions using liquid chromatography tandem mass spectrometry (LC-MS/MS) are also provided for the validated range of 49 to 307 mg/kg. This method has also been shown to be fit for purpose for the determination of theobromine in baking chocolate by both HPLC-UV and LC-MS/MS.

Futtermittel: Probenahme- und Untersuchungsverfahren - Bestimmung von Theobromin in Einzel- und Mischfuttermitteln, einschließlich aus Kakao gewonnenen Bestandteilen, mittels Flüssigchromatographie

Dieses Dokument legt ein Prüfverfahren für die Bestimmung von Theobromin in Einzelfuttermittel oder in Mischfuttermitteln mittels Flüssigchromatographie, gekoppelt mit einem UV Detektor (HPLC UV) im Arbeitsbereich von 27 mg/kg bis 307 mg/kg oder mittels Flüssigchromatographie mit Tandem Massen-spektrometrie (LC MS/MS) im Arbeitsbereich von 49 mg/kg bis 307 mg/kg fest.
Dieses Verfahren wurde unter Verwendung von Ergänzungsmischfuttermittel für erwachsene Hunde und Ergänzungsmischfuttermittel für Pferde vollständig validiert.
Dieses Verfahren gilt ebenfalls als für die Bestimmung von Theobromin in Blockschokolade, entweder mittels HPLC UV  oder LC MS/MS Systeme anwendbar.
Der Arbeitsbereich kann erweitert werden, vorausgesetzt der erweiterte Bereich ist validiert.

Aliments des animaux : Méthodes d'échantillonnage et d'analyse — Détermination par chromatographie en phase liquide de la teneur en théobromine dans les matières premières destinées aux aliments des animaux et dans les aliments composés pour animaux, y compris les ingrédients issus du cacao

Le présent document spécifie une méthode d’essai applicable à la détermination de la teneur en théobromine dans les aliments pour animaux ou les aliments composés par chromatographie en phase liquide couplée à une détection UV (CLHP-UV) dans la gamme de travail de 27 mg/kg à 307 mg/kg ou par chromatographie en phase liquide couplée à une spectrométrie de masse en tandem (CL SM/SM) dans la gamme de travail de 49 mg/kg à 307 mg/kg.
Cette méthode a été intégralement validée avec des aliments composés complémentaires pour chiens adultes et des aliments composés complémentaires pour chevaux.
Cette méthode est également considérée applicable pour déterminer la teneur en théobromine dans le chocolat pâtissier à l’aide de systèmes CLHP-UV ou CL-SM/SM.
La gamme de travail peut être élargie à condition que la gamme élargie soit validée.

Krma: metode vzorčenja in analize - Določevanje teobromina v sestavinah krme in krmnih mešanicah, vključno s pridobljenimi sestavinami iz kakava, s tekočinsko kromatografijo

Metoda v tem dokumentu je uporabna za določanje teobromina v krmni mešanici s tekočinsko kromatografijo z UV-odkrivanjem v preskusnem obsegu 27–307 mg/kg. Ta metoda je bila potrjena z dopolnilno krmno mešanico za odrasle pse in dopolnilno krmno mešanico za konje. Dejanski delovni obseg lahko presega preskušeni obseg. Alternativni pogoji kromatografije s tandemsko masno spektrometrijo s tekočinsko kromatografijo (LC-MS/MS) so prav tako zagotovljeni za potrjeni obseg 49–307 mg/kg. Pokazalo se je tudi, da je ta metoda primerna za določanje teobromina v čokoladi za peko s HPLC-UV in LC-MS/MS.

General Information

Status
Published
Publication Date
22-Oct-2019
Withdrawal Date
29-Apr-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
23-Oct-2019
Due Date
04-Mar-2019
Completion Date
23-Oct-2019

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17270:2020
01-januar-2020
Krma: metode vzorčenja in analize - Določevanje teobromina v sestavinah krme in
krmnih mešanicah, vključno s pridobljenimi sestavinami iz kakava, s tekočinsko
kromatografijo
Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine
in feed materials and compound feed, including cocoa derived ingredients, by liquid
chromatography
Futtermittel: Probenahme- und Untersuchungsverfahren - Bestimmung von Theobromin
in Einzel- und Mischfuttermitteln, einschließlich aus Kakao gewonnenen Bestandteilen,
mittels Flüssigchromatographie
Aliments des animaux : Méthodes d'échantillonnage et d'analyse — Détermination par
chromatographie en phase liquide de la teneur en théobromine dans les matières
premières destinées aux aliments des animaux et dans les aliments composés pour
animaux, y compris les ingrédients issus du cacao
Ta slovenski standard je istoveten z: EN 17270:2019
ICS:
65.120 Krmila Animal feeding stuffs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN 17270:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 17270:2020

---------------------- Page: 2 ----------------------
SIST EN 17270:2020


EN 17270
EUROPEAN STANDARD

NORME EUROPÉENNE

October 2019
EUROPÄISCHE NORM
ICS 65.120
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Determination of theobromine in feed materials and
compound feed, including cocoa derived ingredients, by
liquid chromatography
Aliments des animaux : Méthodes d'échantillonnage et Futtermittel: Probenahme- und
d'analyse - Détermination par chromatographie en Untersuchungsverfahren - Bestimmung von
phase liquide de la teneur en théobromine dans les Theobromin in Einzelfuttermitteln, vor allem aus
matières premières destinées aux aliments des Kakao gewonnen sowie in Mischfuttermitteln mittels
animaux et dans les aliments composés pour animaux, Flüssigchromatographie
y compris les ingrédients issus du cacao
This European Standard was approved by CEN on 28 July 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17270:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 17270:2020
EN 17270:2019 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus .
...

SLOVENSKI STANDARD
oSIST prEN 17270:2018
01-oktober-2018
.UPDPHWRGHY]RUþHQMDLQDQDOL]H'RORþHYDQMHWHREURPLQDYVHVWDYLQDKNUPHLQ
NUPQLKPHãDQLFDKSUHGYVHPL]NDNDYDSULGREOMHQLKVHVWDYLQVWHNRþLQVNR
NURPDWRJUDILMR.RPSOHPHQWDUQLHOHPHQW
Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine
in feed materials and compound feed, including cocoa derived ingredients, by liquid
chromatography - Complementary element
Futtermittel: Probenahme- und Untersuchungsverfahren - Bestimmung von Theobromin
in Einzelfuttermitteln, vor allem aus Kakao gewonnen sowie in Mischfuttermitteln mittels
Flüssigchromatographie
Aliments des animaux : Méthodes d'échantillonnage et d'analyse - Détermination par
chromatographie en phase liquide de la teneur en théobromine dans les matières
premières destinées aux aliments des animaux et dans les aliments composés pour
animaux, y compris les ingrédients issus du cacao
Ta slovenski standard je istoveten z: prEN 17270
ICS:
65.120 Krmila Animal feeding stuffs
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
oSIST prEN 17270:2018 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 17270:2018

---------------------- Page: 2 ----------------------
oSIST prEN 17270:2018


DRAFT
EUROPEAN STANDARD
prEN 17270
NORME EUROPÉENNE

EUROPÄISCHE NORM

August 2018
ICS 65.120
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Determination of theobromine in feed materials and
compound feed, including cocoa derived ingredients, by
liquid chromatography - Complementary element
Aliments des animaux : Méthodes d'échantillonnage et Futtermittel: Probenahme- und
d'analyse - Détermination par chromatographie en Untersuchungsverfahren - Bestimmung von
phase liquide de la teneur en théobromine dans les Theobromin in Einzelfuttermitteln, vor allem aus
matières premières destinées aux aliments des Kakao gewonnen sowie in Mischfuttermitteln mittels
animaux et dans les aliments composés pour animaux, Flüssigchromatographie
y compris les ingrédients issus du cacao
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 327.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17270:2018 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 17270:2018
prEN 17270:2018 (E)
Contents
Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions .
...

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