Animal feeding stuffs - Methods of sampling and analysis - Determination of benzoic and sorbic acid by High Performance Liquid Chromatography (HPLC)

This document specifies a method for the determination of benzoic acid and sorbic acid in animal feeding stuffs by high-performance liquid chromatography method with ultraviolet detection (HPLC-UV).
The method is intended to be used for the determination of benzoic acid and sorbic acid as active substances in feed additives, premixtures, feed materials and compound feed and for benzoic acid in water. This method determines the total extractable concentration of these organic acids and their salts.
It is advisable that the working range of the method is determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. It is advisable that a working range between 5 mg/l and 100 mg/l is accessible.
The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 9,0 %.
On the basis of the referred working range, sample weight and extraction volume, limits of quantification (LOQ), as calculated (Table 1) on the basis of a wavelength of 230 nm, can be achievable.

Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von Benzoesäure und Sorbinsäure mittels Hochleistungs-Flüssigchromatographie (HPLC)

Dieses Dokument legt ein Verfahren zur Bestimmung von Benzoesäure und Sorbinsäure in Futtermitteln mittels Hochleistungs-Flüssigchromatographie mit Ultraviolett Detektion (HPLC UV, en: high performance liquid chromatography with ultraviolet detection) fest.
Das Verfahren ist für die Verwendung zur Bestimmung von Benzoesäure und Sorbinsäure als Wirkstoffe in Futtermittelzusatzstoffen, Futtermittel Ausgangserzeugnissen, Vormischungen, Mischfuttermitteln und für Benzoesäure in Wasser vorgesehen. Dieses Verfahren ermittelt die extrahierbare Gesamtkonzentration dieser organischen Säuren und ihrer Salze.
Dem Anwender dieser Norm wird empfohlen, den Arbeitsbereich des Verfahrens für jede organische Säure zu ermitteln. Der untere Grenzwert des Arbeitsbereiches hängt von der Matrix und den aufgetretenen Störungen ab. Es wird empfohlen, dass ein Arbeitsbereich zwischen 5 mg/l und 100 mg/l eingehalten wird.
Das Verfahren wurde im Rahmen einer Ringversuchsstudie bei Konzentrationen von 0,02 % bis 9,0 % erfolg¬reich geprüft.
Auf der Grundlage des zugehörigen Arbeitsbereichs, der Probenmasse und des Extraktionsvolumens können Bestimmungsgrenzen (LOQ) (en: limits of quantification) wie auf der Grundlage einer Wellen¬länge von 230 nm berechnet (Tabelle 1) erreichbar sein.

Aliments des animaux - Méthodes d’échantillonnage et d’analyse - Dosage de l’acide benzoïque et de l’acide sorbique par chromatographie liquide à haute pression (CLHP)

Le présent document spécifie une méthode de dosage de l’acide benzoïque et de l’acide sorbique dans les aliments pour animaux, par chromatographie liquide à haute performance avec détecteur UV (CLHP UV).
La méthode est destinée à permettre le dosage de l’acide benzoïque et de l’acide sorbique présents comme substances actives dans les additifs pour l’alimentation animale, les prémélanges, les matières premières pour aliments et les aliments composés pour animaux, ainsi que le dosage de l’acide benzoïque dans l’eau. Cette méthode détermine la concentration totale extractible de ces acides organiques et de leurs sels.
Il est recommandé à l’utilisateur de la présente norme de déterminer le domaine de mesure de la méthode pour chaque acide organique. La limite inférieure du domaine de mesure dépend de la matrice et des interférences rencontrées. Il convient de pouvoir atteindre un domaine de mesure compris entre 5 mg/l et 100 mg/l.
La méthode a été soumise à essai avec succès lors d’une étude interlaboratoires à des concentrations allant de 0,02 % à 9,0 %.
En s’appuyant sur le domaine de mesure mentionné, le poids de l’échantillon et le volume d’extraction, il est possible d’atteindre les limites de quantification (LQ), telles que calculées (Tableau 1) pour une longueur d’onde de 230 nm.
Tableau 1 - Limites de quantification (LQ) à 230 nm
Acide organique   LQ (mg/kg)
Acide benzoïque   200
Acide sorbique   200

Krma - Metode vzorčenja in analize - Določevanje benzojske in sorbinske kisline s tekočinsko kromatografijo visoke ločljivosti (HPLC)

Ta dokument določa metodo za določevanje benzojske kisline in sorbinske kisline v krmi s tekočinsko kromatografijo visoke ločljivosti z UV-detekcijo (HPLC-UV).
Ta metoda je predvidena za določevanje benzojske kisline in sorbinske kisline kot aktivnih snovi v dodatkih za krmo, premiksih, krmnih mešanicah ter za benzojsko kislino v vodi. Ta metoda določa skupno koncentracijo teh organskih kislin in njihovih soli, ki jo je mogoče ekstrahirati.
Uporabnik tega standarda mora določiti delovni razpon metode za vsako organsko kislino. Spodnja meja delovnega razpona je odvisna od matrice in motenj, ki se pojavijo. Delovni razpon med 5 mg/l in 100 mg/l naj bi bil dosegljiv.
Metoda je bila uspešno preskušena v medlaboratorijski študiji pri koncentracijah od 0,02 % do 9 %.
Na podlagi omenjenega delovnega razpona, teže vzorca in volumna ekstrakta naj bi bile mejne vrednosti kvantifikacije (LOQ) dosegljive, kot so izračunane na podlagi valovne dolžine 230 nm (preglednica 1).

General Information

Status
Published
Publication Date
06-Aug-2019
Withdrawal Date
28-Feb-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
07-Aug-2019
Due Date
23-Jan-2019
Completion Date
07-Aug-2019

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17298:2019
01-oktober-2019
Krma - Metode vzorčenja in analize - Določevanje benzojske in sorbinske kisline s
tekočinsko kromatografijo visoke ločljivosti (HPLC)
Animal feeding stuffs - Methods of sampling and analysis - Determination of benzoic and
sorbic acid by High Performance Liquid Chromatography (HPLC)
Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von
Benzoesäure und Sorbinsäure mittels Hochleistungs-Flüssigchromatographie (HPLC)
Aliments des animaux - Méthodes d’échantillonnage et d’analyse - Dosage de l’acide
benzoïque et de l’acide sorbique par chromatographie liquide à haute pression (CLHP)
Ta slovenski standard je istoveten z: EN 17298:2019
ICS:
65.120 Krmila Animal feeding stuffs
71.040.40 Kemijska analiza Chemical analysis
SIST EN 17298:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 17298:2019

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SIST EN 17298:2019


EN 17298
EUROPEAN STANDARD

NORME EUROPÉENNE

August 2019
EUROPÄISCHE NORM
ICS 65.120; 71.040.40
English Version

Animal feeding stuffs - Methods of sampling and analysis -
Determination of benzoic and sorbic acid by High
Performance Liquid Chromatography (HPLC)
Aliments des animaux - Méthodes d'échantillonnage et Futtermittel - Probenahme- und
d'analyse - Dosage de l'acide benzoïque et de l'acide Untersuchungsverfahren - Bestimmung von
sorbique par chromatographie liquide à haute pression Benzoesäure und Sorbinsäure mittels Hochleistungs-
(CLHP) Flüssigchromatographie (HPLC)
This European Standard was approved by CEN on 17 June 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17298:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 17298:2019
EN 17298:2019 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Reagents . 6
6 Apparatus . 7
7 Sampling . 8
8 Preparation of test sample . 8
9 Procedure.
...

SLOVENSKI STANDARD
oSIST prEN 17298:2018
01-oktober-2018
.UPDPHWRGHY]RUþHQMDLQDQDOL]H'RORþHYDQMHEHQ]RMVNHLQVRUELQVNHNLVOLQHV
WHNRþLQVNRNURPDWRJUDILMRYLVRNHORþOMLYRVWL +3/&
Animal feeding stuffs: Methods of sampling and analysis - Determination of benzoic and
sorbic acid by High Pressure Liquid Chromatography (HPLC)
Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von
Benzoesäure und Sorbinsäure mittels Hochleistungs-Flüssigchromatographie (HPLC)
Aliments des animaux: Méthodes d’échantillonnage et d’analyse - Dosage de l’acide
benzoïque et de l’acide sorbique par chromatographie liquide à haute pression (CLHP)
Ta slovenski standard je istoveten z: prEN 17298
ICS:
65.120 Krmila Animal feeding stuffs
71.040.40 Kemijska analiza Chemical analysis
oSIST prEN 17298:2018 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 17298:2018

---------------------- Page: 2 ----------------------
oSIST prEN 17298:2018


DRAFT
EUROPEAN STANDARD
prEN 17298
NORME EUROPÉENNE

EUROPÄISCHE NORM

August 2018
ICS 65.120; 71.040.40
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Determination of benzoic and sorbic acid by High Pressure
Liquid Chromatography (HPLC)

This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 327.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17298:2018 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 17298:2018
prEN 17298:2018 (E)
Contents
Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Term and definitions . 6
4 Principle . 7
5 Reagents . 7
5.1 Water, complying with grade 2 in accordance with EN ISO 3696:1995 . 7
5.2 Benzoic acid,p.a., minimum 99 % purity . 7
5.3 Sorbic acid, p.a., minimum 99 % purity . 7
5.4 Acetic acid, p.a., ω = 100 % .
...

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