ISO 23920:2020

Cigarettes — Determination of ammonia in cigarette mainstream smoke with an intense smoking regime using ion chromatography

This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 20778 smoking parameters. This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.

Cigarettes — Dosage de l'ammoniac dans le courant principal de la fumée de cigarette avec un régime de fumage intense par chromatographie par échange d’ions

General Information

• Status: Published
• Publication Date: 29-Jun-2020
• ICS: 65.160 - Tobacco, tobacco products and related equipment
• Technical Committee: ISO/TC 126 - Tobacco and tobacco products
• Drafting Committee: ISO/TC 126/WG 10 - Intense smoking regime
• Current Stage: 6060 - International Standard published
• Start Date: 30-Jun-2020
• Due Date: 16-Aug-2020
• Completion Date: 30-Jun-2020

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 23920
First edition
2020-06
Cigarettes — Determination of
ammonia in cigarette mainstream
smoke with an intense smoking
regime using ion chromatography
Cigarettes — Dosage de l'ammoniac dans le courant principal
de la fumée de cigarette avec un régime de fumage intense par
chromatographie par échange d’ions
Reference number
ISO 23920:2020(E)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO 23920:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO 23920:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 2
7 Preparation . 3
7.1 General . 3
7.2 Preparation of solutions . 3
7.2.1 Sulphuric acid, 0,01 M — Impinger solution (trapping system 1) . 3
7.2.2 Sulphuric acid, 0,1 M — Solution C (Ion Chromatography Eluent). 3
7.2.3 MSA 0,003 M — Solution A (Ion chromatography eluent) . 3
7.2.4 Sulphuric acid, 0,01 M — Ammonium standards preparation . 3
7.2.5 Hydrochloric acid, 0,05 M — Solution for GFP impregnation (trapping system 2) 3
7.2.6 Hydrochloric acid, 0,01 M — Extraction solution (trapping system 2) . 3
7.3 Preparation of standards. 3
7.3.1 Primary ammonium stock solution . 3
7.3.2 Calibration standards . 4
8 Sampling . 4
9 Tobacco product preparation . 4
10 Sample generation — Smoking of cigarettes . 4
10.1 General . 4
10.2 Smoking machine setup . 4
10.2.1 General. 4
10.2.2 Trapping system 1 — GFP and impinger traps . 4
10.2.3 Trapping system 2 — Impregnated GFP . 5
10.3 Smoking . 6
11 Sample analysis . 6
11.1 Preparation of sample. 6
11.1.1 Extraction of GFP – Trapping system 1 . 6
11.1.2 Extraction of GFP – Trapping system 2 . 6
11.2 Determination . 6
11.2.1 Ion chromatography operating conditions . 6
11.2.2 Calibration . 7
11.2.3 Calculation . 8
12 Repeatability and reproducibility . 8
12.1 General . 8
12.2 Results of the 2015 collaborative study . 9
13 Report . 9
Annex A (informative) Examples of chromatograms .10
Bibliography .11
© ISO 2020 – All rights reserved iii

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ISO 23920:2020(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

---------------------- Page: 4 ----------------------
ISO 23920:2020(E)

Introduction
1) 2)
The CORESTA Smoke Analytes Sub-Group conducted a survey among its members and determined
that most laboratories used a method involving ion chromatography for the determination of ammonia
in cigarette mainstream smoke. Two alternative trapping systems were used, either with a combination
of glass fibre filter pad followed by impinger traps or with an impregnated GFP followed by a GFP.
[1]
A CORESTA recommended method (CRM) was written on the basis of the results obtained in an
interlaboratory study conducted in 2015 involving 18 laboratories and 8 samples using cigarettes
[2]
manufactured from a range of blend styles . The results demonstrated equivalency of the data
obtained by using both trapping systems. It was observed that the method is not applicable to dark-air
cured cigarettes.
This document is based upon the CRM 83 and includes statistical evaluations carried out according to
[3] [4]
ISO 5725-1 and ISO 5725-2 .
No machine smoking regime can represent all human smoking behaviour.
— It is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document.
— Machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about exposure and risk across brands.
— Smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using ISO standards.
1) Available at: www .coresta .org.
2) Until 2017, the sub-group was known as CORESTA Special Analytes Sub-Group.
© ISO 2020 – All rights reserved v

---------------------- Page: 5 ----------------------
INTERNATIONAL STANDARD ISO 23920:2020(E)
Cigarettes — Determination of ammonia in cigarette
mainstream smoke with an intense smoking regime using
ion chromatography
WARNING — The use of this document involves hazardous materials, operations and equipment.
This document does not purport to address all the safety problems associated with its use. It
is the responsibility of the user of this document to establish appropriate safety and health
practices and determine the applicability of any other restrictions prior to use.
1 Scope
This document specifies a method for the quantification of ammonia by ion chromatography in
mainstream smoke using ISO 20778 smoking parameters.
This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not
applicable for the determination of ammonia in dark-air cured cigarettes.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 8243, Cigarettes — Sampling
ISO 20778, Cigarettes — Routine analytical cigarette smoking machine — Definitions and standard
conditions with an intense smoking regime
ISO 20779, Cigarettes — Generation and collection of total particulate matter using a routine analytical
smoking machine with an intense smoking regime
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4 Principle
Ammonia is collected by passing the mainstream smoke of cigarettes with intense smoking regime
through either:
3)
a) a glass fibre filter pad as specified in ISO 20779 (e.g. Cambridge filter pad, CFP ) followed by
impinger traps containing dilute sulphuric acid (trapping system 1 in this document), or
3) Cambridge filter pad is an example of a suitable product available commercially. This information is given for
the convenience of users of this document and does not constitute an endorsement by ISO of this product.
© ISO 2020 – All rights reserved 1

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ISO 23920:2020(E)

b) an impregnated glass fibre filter pad followed by an untreated glass fibre filter pad (trapping
system 2 in this document).
The glass fibre filter pads are extracted either with the impinger solutions (trapping system 1)
or with dilute hydrochloric acid (trapping system 2). The obtained solutions are analysed by ion
chromatography using an external standard calibration.
5 Apparatus
The usual laboratory apparatus for use in preparation of samples, solutions and standards and, in
particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 20778.
5.2 Impinger trapping system, capable of being connected in series.
5.3 Ion chromatography system, consisting of a
...

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 23920
ISO/TC 126
Cigarettes — Determination of
Secretariat: DIN
ammonia in cigarette mainstream
Voting begins on:
2020­04­01 smoke with an intense smoking
regime using ion chromatography
Voting terminates on:
2020­05­27
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/FDIS 23920:2020(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
©
NATIONAL REGULATIONS. ISO 2020

---------------------- Page: 1 ----------------------
ISO/FDIS 23920:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH­1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/FDIS 23920:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 2
7 Preparation . 3
7.1 General . 3
7.2 Preparation of solutions . 3
7.2.1 Sulphuric acid, 0,01 M — Impinger solution (trapping system 1) . 3
7.2.2 Sulphuric acid, 0,1 M — Solution C (Ion Chromatography Eluent). 3
7.2.3 MSA 0,003 M — Solution A (Ion chromatography eluent) . 3
7.2.4 Sulphuric acid, 0,01 M — Ammonium standards preparation . 3
7.2.5 Hydrochloric acid, 0,05 M — Solution for CFP impregnation (trapping system 2) 3
7.2.6 Hydrochloric acid, 0,01 M — Extraction solution (trapping system 2) . 3
7.3 Preparation of standards. 3
7.3.1 Primary ammonium stock solution . 3
7.3.2 Calibration standards . 4
8 Sampling . 4
9 Tobacco product preparation . 4
10 Sample generation — Smoking of cigarettes . 4
10.1 General . 4
10.2 Smoking machine setup . 4
10.2.1 General. 4
10.2.2 Trapping system 1 — CFP and impinger traps . 4
10.2.3 Trapping system 2 — Impregnated CFP . 5
10.3 Smoking . 6
11 Sample analysis . 6
11.1 Preparation of sample. 6
11.1.1 Extraction of CFP – Trapping system 1 . 6
11.1.2 Extraction of CFP – Trapping system 2 . 6
11.2 Determination . 6
11.2.1 Ion chromatography operating conditions . 6
11.2.2 Calibration . 7
11.2.3 Calculation . 8
12 Repeatability and reproducibility . 8
12.1 General . 8
12.2 Results of the 2015 collaborative study . 9
13 Report . 9
Annex A (informative) Examples of chromatograms .10
Bibliography .11
© ISO 2020 – All rights reserved iii

---------------------- Page: 3 ----------------------
ISO/FDIS 23920:2020(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

---------------------- Page: 4 ----------------------
ISO/FDIS 23920:2020(E)

Introduction
1) 2)
The CORESTA Smoke Analytes Sub-Group conducted a survey among its members and determined
that most laboratories used a method involving ion chromatography for the determination of ammonia
in cigarette mainstream smoke. Two alternative trapping systems were used, either with a combination
of Cambridge filter pad followed by impinger traps or with an impregnated CFP followed by a CFP.
[1]
A CORESTA recommended method (CRM) was written on the basis of the results obtained in an
interlaboratory study conducted in 2015 involving 18 laboratories and 8 samples using cigarettes
[2]
manufactured from a range of blend styles . The results demonstrated equivalency of the data
obtained by using both trapping systems. It was observed that the method was not applicable to dark-
air cured blended cigarettes.
This document is based upon the CRM 83 and includes statistical evaluations carried out according to
ISO 5725­1 and ISO 5725­2.
No machine smoking regime can represent all human smoking behaviour.
— It is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document.
— Machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about exposure and risk across brands.
— Smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using ISO standards.
1) Available at: www .coresta .org.
2) Until 2017, the sub-group was known as CORESTA Special Analytes Sub-Group.
© ISO 2020 – All rights reserved v

---------------------- Page: 5 ----------------------
FINAL DRAFT INTERNATIONAL STANDARD ISO/FDIS 23920:2020(E)
Cigarettes — Determination of ammonia in cigarette
mainstream smoke with an intense smoking regime using
ion chromatography
WARNING — The use of this document involves hazardous materials, operations and equipment.
This document does not purport to address all the safety problems associated with its use. It
is the responsibility of the user of this document to establish appropriate safety and health
practices and determine the applicability of any other restrictions prior to use.
1 Scope
This document specifies a method for the quantification of ammonia by ion chromatography in
mainstream smoke using ISO 20778 smoking parameters.
This method is applicable to an ammonia yield of about 10 µg/cigarette to 40 µg/cigarette. It is not
applicable for the determination of ammonia in dark-air cured cigarettes.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 8243, Cigarettes — Sampling
ISO 20778, Cigarettes — Routine analytical cigarette smoking machine — Definitions and standard
conditions with an intense smoking regime
ISO 20779, Cigarettes — Generation and collection of total particulate matter using a routine analytical
smoking machine with an intense smoking regime
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4 Principle
Ammonia is collected by passing the mainstream smoke of cigarettes with intense smoking regime
through either:
a) a glass fibre filter pad as specified in ISO 20779 (e.g. Cambridge filter pad, CFP) followed by
impinger traps containing dilute sulphuric acid (trapping system 1 in the document), or
b) an impregnated glass fibre filter pad followed by an untreated glass fibre filter pad (trapping
system 2 in the document).
© ISO 2020 – All rights reserved 1

---------------------- Page: 6 ----------------------
ISO/FDIS 23920:2020(E)

The glass fibre filter pads are extracted either with the impinger solutions (trapping system 1)
or with dilute hydrochloric acid (trapping system 2). The obtained solutions are analysed by ion
chromatography using an external standard calibration.
5 Apparatus
The usual laboratory apparatus for use in preparation of samples, solutions and standards and, in
particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 20778.
5.2 Imp
...