ISO/TS 23459:2021

Nanotechnologies — Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism

This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy. This document does not apply to the characterization of conformational changes of disordered proteins.

Nanotechnologies — Évaluation de la structure secondaire des protéines durant une interaction avec des nanomatériaux à l'aide du dichroïsme circulaire ultraviolet

General Information

• Status: Published
• Publication Date: 18-Jan-2021
• ICS: 07.120 - Nanotechnologies
• Technical Committee: ISO/TC 229 - Nanotechnologies
• Drafting Committee: ISO/TC 229/WG 3 - Health, Safety and Environmental Aspects of Nanotechnologies
• Current Stage: 9020 - International Standard under periodical review
• Start Date: 15-Jan-2024
• Completion Date: 15-Jan-2024

Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 23459
First edition
2021-01
Nanotechnologies — Assessment of
protein secondary structure during an
interaction with nanomaterials using
ultraviolet circular dichroism
Nanotechnologies — Évaluation de la structure secondaire des
protéines durant une interaction avec des nanomatériaux à l'aide du
dichroïsme circulaire ultraviolet
Reference number
ISO/TS 23459:2021(E)
©
ISO 2021

---------------------- Page: 1 ----------------------
ISO/TS 23459:2021(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/TS 23459:2021(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 Nanomaterial protein interactions . 2
6 Sample preparation . 3
6.1 General . 3
6.2 Desired properties of the UV-CD quartz cell. 3
6.3 Preparation of protein solution . 3
6.4 Instrumental setting condition . 4
6.5 Recording UV-CD spectra procedure. 4
6.5.1 General. 4
6.5.2 Buffer . 4
6.5.3 Protein sample . 4
6.5.4 Stability of NP suspension in the protein solution . 5
6.6 Preparation of protein-NPs conjugated suspension . 5
6.7 UV-CD spectra measurement .
...

TECHNICAL ISO/TS
SPECIFICATION 23459
First edition
Nanotechnologies — Assessment of
protein secondary structure during an
interaction with nanomaterials using
ultraviolet circular dichroism
Nanotechnologies — Évaluation de la structure secondaire des
protéines durant une interaction avec des nanomatériaux à l'aide du
dichroïsme circulaire ultraviolet
PROOF/ÉPREUVE
Reference number
ISO/TS 23459:2020(E)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO/TS 23459:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii PROOF/ÉPREUVE © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/TS 23459:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 Nanomaterial protein interactions . 2
6 Sample preparation . 3
6.1 General . 3
6.2 Desired properties of the UV-CD quartz cell. 3
6.3 Preparation of protein solution . 3
6.4 Instrumental setting condition . 4
6.5 Recording UV-CD spectra procedure. 4
6.5.1 General. 4
6.5.2 Buffer . 4
6.5.3 Protein sample . 4
6.5.4 Stability of NP suspension in the protein solution . 5
6.6 Preparation of protein-NPs conjugated suspension . 5
6.7 UV-CD spectra measurement .
...