Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 2: Enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization

This document specifies a method for the direct enumeration of potentially enteropathogenic V. parahaemolyticus (tdh and/or trh positive) and/or the enumeration of total V. parahaemolyticus in seafood.

Microbiologie de la chaîne alimentaire — Méthode horizontale pour la détermination des Vibrio spp. — Partie 2: Dénombrement de Vibrio parahaemolyticus total et potentiellement entéropathogène dans les fruits de mer, par hybridation des acides nucléiques

General Information

Status
Published
Publication Date
26-Oct-2020
Current Stage
9093 - International Standard confirmed
Completion Date
04-Mar-2024
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Relations

Effective Date
06-Jun-2022

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Technical specification
ISO/TS 21872-2:2020 - Microbiology of the food chain -- Horizontal method for the determination of Vibrio spp.
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ISO/PRF TS 21872-2:Version 12-sep-2020 - Microbiology of the food chain -- Horizontal method for the determination of Vibrio spp.
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Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 21872-2
First edition
2020-10
Microbiology of the food chain —
Horizontal method for the
determination of Vibrio spp. —
Part 2:
Enumeration of total and
potentially enteropathogenic Vibrio
parahaemolyticus in seafood using
nucleic acid hybridization
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la détermination des Vibrio spp. —
Partie 2: Dénombrement de Vibrio parahaemolyticus total et
potentiellement entéropathogène dans les fruits de mer, par
hybridation des acides nucléiques
Reference number
ISO/TS 21872-2:2020(E)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO/TS 21872-2:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/TS 21872-2:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Plating . 2
4.3 Preparation of membranes . 2
4.4 Hybridization. 2
4.5 Detection . 2
5 Culture media and reagents . 3
5.1 General . 3
5.2 Hybridization reagents . 3
5.2.1 Hybridization buffer . 3
5.2.2 Digoxigenin-labelled oligoprobes . 3
5.2.3 Control materials .
...

TECHNICAL ISO/TS
SPECIFICATION 21872-2
First edition
Microbiology of the food chain —
Horizontal method for the
determination of Vibrio spp. —
Part 2:
Enumeration of total and
potentially enteropathogenic Vibrio
parahaemolyticus in seafood using
nucleic acid hybridization
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la détermination des Vibrio spp. —
Partie 2: Dénombrement de Vibrio parahaemolyticus total et
potentiellement entéropathogène dans les fruits de mer, par
hybridation des acides nucléiques
PROOF/ÉPREUVE
Reference number
ISO/TS 21872-2:2020(E)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO/TS 21872-2:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii PROOF/ÉPREUVE © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/TS 21872-2:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Plating . 2
4.3 Preparation of membranes . 2
4.4 Hybridization. 2
4.5 Detection . 2
5 Culture media and reagents . 3
5.1 General . 3
5.2 Hybridization reagents . 3
5.2.1 Hybridization buffer . 3
5.2.2 Digoxigenin-labelled oligoprobes . 3
5.2.3 Control materials .
...

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