SIST EN 4159:2012
(Main)Aerospace series - Paints and varnishes - Determination of resistance to microbial growth
Aerospace series - Paints and varnishes - Determination of resistance to microbial growth
This European Standard specifies a method to assess the ability of biocide-containing coatings to prevent the germination of conidiospores of certain fungi known to be capable of proliferating in fuel systems for aerospace applications.
Luft- und Raumfahrt - Anstrichstoffe - Bestimmung der Widerstandsfähigkeit gegen Schimmelwachstum
Diese Europäische Norm legt ein Verfahren zum Beurteilen von biozidhaltigen Beschichtungen zur Vermeidung der Keimung von Konidiosporen bestimmter Pilze fest, die für ihre starke Vermehrung in Kraftstoffanlagen bekannt sind, die in der Luft- und Raumfahrt angewendet werden.
Série aérospatiale - Peintures et vernis - Détermination de la résistance à l'action des microorganismes
Aeronavtika - Barve in laki - Ugotavljanje odpornosti proti razvoju plesni
Ta evropski standard določa metodo za oceno sposobnosti lakov, ki vsebujejo biocid, za preprečevanje kalitve trosov nekaterih vrst gliv, za katere je znano, da se lahko razvijejo v sistemih za gorivo za uporabo v aeronavtiki.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Aeronavtika - Barve in laki - Ugotavljanje odpornosti proti razvoju plesniLuft- und Raumfahrt - Anstrichstoffe - Bestimmung der Widerstandsfähigkeit gegen SchimmelwachstumSérie aérospatiale - Peintures et vernis - Détermination de la résistance à l'action des microorganismesAerospace series - Paints and varnishes - Determination of resistance to microbial growth49.040Prevleke in z njimi povezani postopki, ki se uporabljajo v letalski in vesoljski industrijiCoatings and related processes used in aerospace industryICS:Ta slovenski standard je istoveten z:EN 4159:2011SIST EN 4159:2012en01-februar-2012SIST EN 4159:2012SLOVENSKI
STANDARD
SIST EN 4159:2012
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 4159
December 2011 ICS 49.040 English Version
Aerospace series - Paints and varnishes - Determination of resistance to microbial growth
Série aérospatiale - Peintures et vernis - Détermination de la résistance à l'action des microorganismes
Luft- und Raumfahrt - Beschichtungsstoffe - Bestimmung der Widerstandsfähigkeit gegen Schimmelwachstum This European Standard was approved by CEN on 27 August 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 4159:2011: ESIST EN 4159:2012
EN 4159:2011 (E) 2 Contents Page Foreword .3 Introduction .4 1 Scope .5 2 Terms and definitions .5 3 Principle .5 4 Apparatus .5 5 Specimen .6 6 Procedure .6 6.1 Test methods .6 6.1.1 Preparation of agarose ++++ glucose gel .6 6.1.2 Application of gel to coating .6 6.1.3 Preparation of spore suspension .6 6.1.4 Application of suspension to gel and storage at 25 °C .7 6.1.5 Microscopic examination .7 6.1.6 Time course of germination .7 7 Designation .8 8 Test report .9 Bibliography . 10
SIST EN 4159:2012
EN 4159:2011 (E) 3 Foreword This document (EN 4159:2011) has been prepared by the Aerospace and Defence Industries Association of Europe - Standardization (ASD-STAN). After enquiries and votes carried out in accordance with the rules of this Association, this Standard has received the approval of the National Associations and the Official Services of the member countries of ASD, prior to its presentation to CEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2012, and conflicting national standards shall be withdrawn at the latest by June 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. SIST EN 4159:2012
EN 4159:2011 (E) 4 Introduction Certain fungi are known to be capable of proliferating in fuel systems which can cause corrosion and blockage. Conidiospores are the dispersal form of these fungi. Germination of conidia is the first stage in proliferation of the fungus. If the conidiospore cannot germinate, there can be no proliferation and no blockage of fuel lines, ducts etc. This method should be performed only by persons qualified in the microbiology of fungi. The standard can be used to assess the effectiveness of new candidate coating systems in inhibiting microbial (fungal) growth. SIST EN 4159:2012
EN 4159:2011 (E) 5 1 Scope This European Standard specifies a method to assess the ability of biocide-containing coatings to prevent the germination of conidiospores of certain fungi known to be capable of proliferating in fuel systems for aerospace applications. 2 Terms and definitions For the purposes of this document, the following terms and definitions apply. 2.1 Conidiospores single-celled structures produced by the mycelial [mould] form of the fungus NOTE Conidiospores are spherical or nearly spherical resting cells, i.e. cells which may be dispersed readily but which do not proliferate. However, conidiospores may germinate if they encounter suitable conditions of moisture and nutrients. On germination, a conidiospore produces a long tube-like outgrowth which then forms dense branching structures [mycelia] which may block fuel ducts etc. A suitable coating will prevent germination of conidiospores. A coating which prevents germination of conidiospores is considered to have fungistatic activity. This fungistatic activity may be assessed quantitatively by assessing the success rate of germination of conidiospores under standard conditions (see below) to determine whether the test coating delays or prevents germination of conidiospores when compared with a coating which is known to possess no fungistatic activity. Laboratories which undertake work to this method should first obtain the test fungi (see 5) and perform control experiments to satisfy themselves that they can follow the process of germination of conidiospores. These initial experiments may be performed by placing the agarose gel (see below) on the surface of sterile plastic petri dishes rather than on the surface of coated test panels, as is done in the present method. 3 Principle 3.1 Conidiospores are placed on a gel within a few millimetres of the panel/coating under test. Under the test conditions a high proportion of these conidia germinate [begin growth] rapidly unless some material in the coating diffuses through the gel and prevents germination. 3.2 The success rate of germination, after any given interval of exposure to the coating, is expressed as the number of cells that have germinated divided by the number of cells examined (germinated + nongerminated). The success rate of germination is determined from time to time, beginning when the conidiospores are first exposed to the coating under test. Examination is made using a microscope [100×]. This allows ready distinction between ungerminated conidiospores [approximately spherical] and the long filamentous outgrowth that is the result of germination. 3.3 The results obtained with conidiospores exposed to test coatings are to be compared with results of conidiospores exposed to coatings that contain no inhibitor. 4 Apparatus 4.1 Incubator, capable of maintaining (25 ± 1) °C. 4.2 Autoclave suitable for
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